Red One

Red One™ is a fully automated microbiology platform that detects stained single cells based on their metabolic activity (esterases).
Based on the new generation of solid phase cytometry technology, Red One™ detects viable cells by using advanced image processing techniques to track and analyze the assimilation of staining agents by the targeted cells (enzymatic labeling). Suited for any lab setup, Red One™ integrates easily into a safety cabinet and has proven a highly reliable detection capacity of individual cells by monitoring their staining kinetics.

Red one has 3 proven applications:

1. Direct Viable count – results in 10 minutes
The Red One™ Direct Viable Count application provides a direct analysis within 10 minutes for the total viable cell count, for samples ranging from 10µL to 250mL.
This application has demonstrated consistency with traditional 5-day counts on R2A agar plates for water loops.
Other applications include seed lot enumeration (ex: mycobacteria), in-process or environmental testing, and more.

2. Bioburden – results in 4 hrs
The Red One™ Bioburden application is designed for the quantification of total aerobic mesophilic viable flora, including fungi and spores.
After the initial sample filtration onto the cap, an activation medium is added, followed by a 4 hours activation step to restore the metabolic activity of the microorganims, making it compliant with staining and detection.

3. Sterility Analysis- results in 96 hrs
The Red One™ Sterility Sample & Analysis application strictly relies on compendial sample preparation in a standard double-canister device. After a 96-hour incubation period, a small volume from the canister, typically ranging from 1 to 10mL, is sampled and analyzed using Red One™.

The Time to Result (TTR) is 96 hours and Red One™ delivers a qualitative result (presence/absence). This short TTR is achieved through the system’s unique capability to detect single microbial cells. In comparison, traditional visual inspection methods require a significantly higher microbial population (typically on the order of 108 cells) for confirmation of a positive result.